Background: The aim of this study is to measure and compare the dynamic thiol/disulphide homeostasis of patients with basal cell carcinoma and healthy subjects with a newly developed and original method.Objective: Thirty four patients attending our outpatient clinic and clinically and histopathologically diagnosed as nodular basal cell carcinoma, and age and gender matched 30 healthy individuals have been involved in the study. Thiol/disulphide homeostasis tests have been measured with a novel automatic spectrophotometric method developed and the results have been compared statistically.Results: Serum native thiol and disulphide levels in the patient and control group show a considerable variance statistically (p=0.028, 0.039, respectively). Total thiol levels do not reveal a considerable variation (p=0.094). Disulphide/native thiol ratios and native thiol/total thiol ratios also show a considerable variance statistically (p=0.012, 0.013, 0.010, respectively).Conclusions: Thiol disulphide homeostasis in patients with basal cell carcinoma alters in the way that disulphide gets lower and thiols get higher. Thiol/disulphide level is likely to have a role in basal cell carcinoma pathogenesis.