Usage of soluble soy protein on enzymatically hydrolysis of apple pomace for cost-efficient bioethanol production

DEMİRAY E., Kut A., ERTUĞRUL KARATAY S., DÖNMEZ G.

Fuel, vol.289, 2021 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 289
  • Publication Date: 2021
  • Doi Number: 10.1016/j.fuel.2020.119785
  • Journal Name: Fuel
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Biotechnology Research Abstracts, Chemical Abstracts Core, Communication Abstracts, INSPEC, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Keywords: Apple pomace, Soluble soy protein, Bioethanol, Enzymatic hydrolysis, Biomass loading
  • Ankara Yıldırım Beyazıt University Affiliated: Yes

Abstract

© 2020 Elsevier LtdIn this study, bioethanol production and the effect of soluble soy protein on enzymatic hydrolysis of apple pomace as a cheap additive investigated. For this purpose essential parameters for fermentation such as biomass loading (5%, 10%, 20%), different media compositions (the whole slurry and washed fraction), enzyme loadings (15, 30, 60, 120 FPU/g substrate), fermentation time (24, 48 and 72 h) and soluble soy protein concentrations (20, 40, 80, 160 mg/g cellulose) were optimised. The positive effect of soluble soy protein on enzymatic hydrolysis and bioethanol production was observed. The highest sugar concentration without soy protein addition was found as 155 g/L in the presence of 120 FPU and S. cerevisiae produced 52.4 g/L bioethanol. On the other hand, when the enzyme concentration decreased two times (60 FPU) and supplemented with 80 mg/g cellulose soluble soy protein, sugar concentration increased to 176 g/L, and S. cerevisiae produced 53.1 g/L bioethanol. This study indicates that apple pomace is a suitable raw material for bioethanol production, and soluble soy protein can be evaluated for more economical bioethanol production.