Comparison of Total Phenolic and Total Flavonoid Contents of Different Solvent Extracts of Achillea sintenisii and Centaurea depressa

Asteraceae is known as one of the largest plant families with thousands of plant species. The genus Centaurea is the largest member of the broader Asteraceae family. The genus Centaurea is represented in Turkey with 187 taxa, 114 of which are endemic. Various species of Centaurea are used as herbal remedies for their digestive, tonic, expectorant, anti-pyretic, and antidiarrheal effects in traditional medicine. Achillea species are represented by approximately 140 species in the world. In folk medicine, these species are used as herbal remedies due to their analgesic, antispasmodic, antiinflammatory digestive and wound healing effects. The aim of this study is to compare the total phenolic and flavonoid contents of various solvent extracts obtained from the aerial parts of Achillea sintenisii and Centaurea depressa. The total phenolic concentration was measured using the Folin Ciocalteu method. Absorbance of the mixtures was determined at 750 nm relative to a prepared blank using Shimadzu (UV-1240) spectrophotometer. The phenolic content determination, gallic asid was used as the standard. Total flavonoid concentration was measured using a aluminum chloride colorimetric assay. Absorbance of the mi􏰓tures was determined at 415 nm relative to a prepared blank using Shimadzu (UV-1240) spectrophotometer. Quercetin was used as a standard in the determination of the flavonoid content. Total phenolic content of plant extracts ingredient contents 3, 053 and 9, 035 varied between them. Total flavonoid content of plant extracts ingredient contents 3, 55 and 12, 25 varied between them.